bacteria culture media ppt

what‘s culture?. culture consists of the beliefs, behaviors, objects, and, Politics, Culture, and Media: - A preliminary case study of contemporary chinese diaspora communities tao yang rutgers, Media & Culture - Dr.inas a.hamid. what do these words mean to you?. Clipping is a handy way to collect important slides you want to go back to later. Media and Youth Culture - . E.g. Several life threating infections can be diagnosed with the appropriate use of Bacterial culture Media, Bacterial CultureMediabasics Dr.T.V.Rao MD, Agar – Agar • Solid medium is made by adding Agar • Agar is obtained from Sea weeds New Zealand agar is more • Agar contain long chain poly saccharides.Inoranic salts and protein like substance • Melts at 980c and sets at 420c, Agar - Agar • Complex polysaccharide • Used as solidifying agent for culture media in Petri plates, slants, and deeps • Generally not metabolized by microbes • Liquefies at 98°C • Solidifies ~42°C • Dr.T.V.Rao MD’s ‘e’ learning series, Media and Culture • Media: Nutrients (agar, pH indicators, proteins and carbohydrates) used to grow organisms outside of their natural habitats • Culture: The propagation of microorganisms using various media, Culture media • Used to grow bacteria • Can be used to: • Enrich the numbers of bacteria • Select for certain bacteria and suppress others • Differentiate among different kinds of bacteria. See our User Agreement and Privacy Policy. Sorbital • Sugar medium contain 1% sugar • Durham’s tube indicates production of gas • Hiss Serum sugars apart from sugar , serum is added. • If more than one species or type of organism are present each requires to be carefully separated or isolated in pure culture. culture media. Key Terms. The major exception is the use of filtration for the sterilization of animal cell culture. media- lb broth. 2. Tryptic Soy Agar (TSY) is a good all-purpose medium commonly used to grow bacteria in the microbiology laboratory. • Charcoal to neutralize certain bacterial inhibitors to Gonococci, Hiss Serum SugarsSugar Medium with Serum enrichment, Anaerobic Medium • Robertson’s cooked meat medium • Thiglyclolate liquid medium, Anaerobic Culture Methods Anaerobic jar • Anaerobic jar Figure 6.5, Sabouraud's Dextrose agar commonly used Fungal Isolation Medium, Sabouraud's Dextrose Agar Dextrose - 4 gm% Neopeptone - 1 gm% Agar - 1.5 gm% Distilled water - 100 ml Dissolve the ingredients by heating in a water bath, cool and adjust pH to 5.4 Autoclave and dispense 20 ml amount in test tubes Use: For the cultivation of Fungi, Robertsons’cooked Meat Medium • Place meat in 1 ounce bottles to the depth of 2.5 cms and cover it with 15 ml of broth • Autoclave at 1210 c for 20 min • After sterilization, adjust the pH to 7.5 • Use: To cultivate the anaerobic bacteria, Lowenstein Jensen Medium - cultivation of Mycobacterium tuberculosis, Lowenstein-Jensen’s medium Mineral salt soln - 600mlMalachite green soln - 20ml(2gm% in D.water)Beaten egg - 1000ml(20-22 eggs) Mix the above Distribute in Mc Cartney bottles Sterilize by Inspissation Use: To cultivate Mycobacteria, © 2020 SlideServe | Powered By DigitalOfficePro, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. Powerpoint presentation. Figure: TSI Agar slant tubes: The agar triple-sugar iron is one of the culture media used for the differentiation of most enterobacteria. Culture and Medium • Culture is the term given to microorganisms that are cultivated in the lab for the purpose of identifying and studying them. Bacterial Culture Methods ppt 1. Lec. These media contain all the elements that most bacteria need for growth and are non-selective, so they are used for the general cultivation and maintenance of bacteria kept in laboratory-culture collections. It should be recognized that inoculation of culture media with bacteria, deliberately or accidentally, leads to very great numbers of organisms being produced. Presentation Summary : Media & Culture. bacterial nutrition and the design of culture media. mohammad rhbar (phd) dep. Culture media can vary in many ingredients allowing the media to select for or against microbes. Presentation Summary : CULTURE MEDIA. Now customize the name of a clipboard to store your clips. Isolating a bacterium from sites in body normally known to be sterile is an indication of its role in the disease process. dr.t.v.rao md. In situations where preparation is uneconomic in time, prepared, sterilized media (liquid and solid) are available from the major school science equipment suppliers. • Medium is the term given to the combination of ingredients that will support the growth and cultivation of microorganisms by providing all the essential nutrients required for the growth (that is, multiplication) in order to cultivate these microorganisms in large numbers to study them. No public clipboards found for this slide, BSc sp. View culture_media.ppt from BIO 3110 at University of Guyana. 1183. selective enrichment. Specific Media • Defined media are media composed of pure ingredients in carefully measured concentrations dissolved in double distilled water i.e., the exact chemical composition of the medium is known. Culture Media - . Heat: Heat is the most important and widely used method. Different types of media are used for growing different types of cells. Types of microbial media. BACTERIAL CULTURE METHODS BASICS Dr.T.V.Rao MDDR.T.V.RAO MD 1. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Culture media • Used to grow bacteria • Can be used to: • Enrich the numbers of bacteria • Select for certain bacteria and suppress others • Differentiate among different kinds of bacteria Culture and Medium • Culture is the term given to microorganisms that are cultivated in the lab for the purpose of identifying and studying them. Precautions in the use and disposal of prepared media. There are general media for screening culture and even that varies with the source of the specimen and suspected organisms, There are sold media and liquid media. In comparison with defined media, which are good for growing picky bacteria, complex media can be thought of as a crowd-pleaser, suitable for growing many different types of less fastidious microbes. overview. microbiology and virology; 3 credit hours atta- ur - rahman school of applied, bacterial Culture methods BASICS - . Simple or basal media: Include nutrient broth and peptone water; used routinely to isolate and culture a variety of bacteria in a molecular biology research laboratory; Complex media: Contain mixture of a variety of nutrients; the exact composition of amino acid source is not defined.Examples include chocolate agar, MacConkey agar, Lowenstein-Jensen medium Culture media - . of microbiology , iranian reference health laboratory. Culturing bacteria is also the initial step in studying Plant Tissue Culture Media - . Based on substitution of glucose in tryptone –yeast extract – glucosemediumbyNa-gluconateorglycerol,twonewculture media were developed for mass cultivation of the commonly usedplantgrowth-promotingbacteriumAzospirillum sp. media, (2) Enriched media, (3) Selective media, (4) Indicator media, (5) Transport media, and (6) Storage media. BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media. today’s menu . BACTERIAL NUTRITION AND THE DESIGN OF CULTURE MEDIA• Based on bacterial metabolism*• Culture pH• Culture oxidation- reduction potential.• High concentrations of any organisms are potentially hazardous and must be disposed of safely by approved methods. A pure culture may be isolated by the use of special media with specific chemical or physical agents that allow the enrichment or selection of one organism over another. Similarly if the culture media is different from the previous culture then duration is long because bacteria takes some more time to adjust in the new media. in agriculture at sabaragamuwa university of sri lanka. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined. Log phase or exponential phase: Create stunning presentation online in just 3 steps. = the whole range, Culture, Media & Deception - . See our Privacy Policy and User Agreement for details. media & culture. What is culture medium • The food material or substances required for growing microorganisms in vitro (outside the body) is called culture medium. Serum for medium can be obtained from animal blood but must be filtered through membrane or seitz filter before use. Osmium (ppt) and 187 os/ 188 os compositions in the culture media. Get powerful tools for managing your contents. at least 500 different types contained in test tubes, Culture and Media - . Some infectious diseases, such as obligate intracellular pathogens (i.e., those … Bacterial Cultivation PPT. The simplest artificial media which are commonly used in the laboratories are Nutrient Broth medium & Nutrient Agar Medium, also known as the Basal Media. Sugar Medium • Sugar medium contain 1% sugar • Durham’s tube indicates production of gas • Hiss Serum sugars apart from sugar , serum is added. Hospital Environmental Cleaning & Disinfection, Procedures & Practices, BEST PRACTICES IN DIAGNOSTIC MICROBIOLOGY, JOURNAL CLUBin medicinepreparation and presentation, CRISIS IN INDIAN MEDICAL EDUCATION Time to Verify, WHONET DOCUMENTATION IN ANTIBIOTIC POLICY. WORK PLACE CHALLENGES - WE CAN FIGHT BACK? Typically, they contain a simple sugar as the carbon and energy source, an inorganic nitrogen source, various mineral salts and if necessary growth factors (purified amino acids, vitamins, purines and pyrimidines, Need for Culture Media • It is usually essential to obtain a culture by grwoing the organism in an artificial medium. Culture Media Introduction of microbes into culture medium Culture: Microbes growing in/on culture medium A pure culture contains only one species or strain Mixed culture contains several species Contaminated culture contains unwanted species of organisms 12/30/13 Dr. Shyamal Kr Paul, Culture media 3 ADVERTISEMENTS: The culture media (nutrients) consist of chemicals which support the growth of culture or microorganisms. culture: The process of growing a bacterial or other biological entity in an artificial medium. BACTERIAL CULTURE METHODS BASICS Dr.T.V.Rao MDDR.T.V.RAO MD 1 2. 1. 3. These rules cannot be applied to all infectious diseases. open culture: A continuous culture where periodically some of the bacterial culture is removed and added to fresh sterile medium. 1. If you continue browsing the site, you agree to the use of cookies on this website. Use enriched, A pure culture theoretically contains a single bacterial species. A growth medium or culture medium is a solid, liquid or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation, or small plants like the moss Physcomitrella patens. Dehydrated culture media lab m. Single versus sequential culture medium: which is better at. Transport Medium • Stuart’s medium contain reducing agents to prevent oxidation. Sugar Medium • Sugars are fermenting substances • Monosaccharide – peptone, arabinose,xylose and hexose's, dextrose and mannose • Disaccharides Sucrose and Lactose • Polysaccharides – Starch and Inulin • Alcohols – Glycerol. College of health sciences-HMU Lab 5 2. Enrichment and Isolation The most common growth media for microorganisms are nutrient broths and agar plates; specialized media … Examples: Nutrient broth, nutrient agar and peptone water. Distribute into sterile test tube containing inverted Durham’s tubes to detect gas production and steam for 30 min Use: To test the fermenting ability of an organism, Carbohydrate media • Peptone water – 100 ml, Desired sugar 1 gm% and Andrade's indicator – 0.005% soln(1ml) • Dissolve the desired carbohydrate in peptone water and steam for 30 min or sterilize by filtration. World's Best PowerPoint Templates - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. There are a number of procedures available for the isolation of pure cultures from mixed populations. Culture media (types, preparation & sterilization) ppt video online. preenrichment. The method for the preparation of basic microbiology media is given below. joey f. george florida state university. If you continue browsing the site, you agree to the use of cookies on this website. Broth: A liquid medium. on solid culture media.This kit designed to educate students inthe lag, log, stationary and decline phases of the bacterial growth cycle and how to monitor the various stages. a culture medium (media, plural) is nutrient material prepared in the laboratory for the growth of. They'll give your presentations a professional, memorable appearance - the kind of sophisticated look that today's audiences expect. Microbes can use the nutrients of culture media as their food is necessary for cultivating them in vitro. vy_32_inovace_aj3r0201. in a bottle with a cap, dissolve 2.5, Culture Media - . babitha elias. For sterilization, the type of heat, time of application and temperature required to ensure destruction of all … other media includes billboards, magazines, radios, newspapers, books.in 1991, playboy, Differential Medium Mac Conkey's agar, Sabouraud's Dextrose agar commonly used Fungal Isolation, Lowenstein Jensen Medium - cultivation of Mycobacterium. Looks like you’ve clipped this slide to already. Bacterial culture media One of the most important reasons for culturing bacteria in vitro is its utility in diagnosing infectious diseases. based on, Culture media Definition :- 1-Synthetic media : 2-Routine culture media: Routine culture media - Common ingredients :-. listeria half fraser broth base cat. Periodic Transfer to Fresh Media Strains can be maintained by periodically preparing a fresh culture from the previous stock culture. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. nutrient agar. Glucose or glycerol are often used as carbon sources, and ammonium salts or nitrates as inorganic nitrogen sources in culture media. logical basis. Types of culture media Prepared by Samira fattah Assis. 105 Bacterial Culture Media MICROBIOLOGY MODULE Microbiology Notes Neisseria sp. You can change your ad preferences anytime. microbiological culture. Media lacking an amino acid, such as proline in conjunction with E. coli unable to synthesize it, were commonly used by geneticists before the emergence of genomics to map bacterial chromosomes. • Several organism need the determination of Antibiotic sensitivity pattern for optimal antibiotic selection, Basic requirements of culture media Nutrients - Energy source - Carbon source - Nitrogen source Mineral salts – Sulphate, phosphates, chlorides & carbonates of K, Mg & Ca. Culture: A bacterial growth in media. Media. Bacterial Media And Culture Collecting Clinical ... 548721 PPT. Agar plate: Round culture dish with semi-solid nutrient media.Several Terms Slant: Tube with semi-solid media, with a slanted surface. Isolation Culture Media An isolation culture medium is a simple agar containing solid medium that allows the growth of microorganisms in the direction of the streaks. These media provide all the basic components that are required by the bacterial cell for the rapid growth in the laboratory. by mgr. Define and use aseptic technique in microbial culture and media preparation. Wall-less bacteria that replicate L forms * Naturally Wall-less Genus Mycoplasma * Pili (fimbriae) hair-like projections of the cell sexual conjugation adhesion to host epithelium * Capsules and slime layers outside cell envelope well defined: capsule not defined: slime layer or … Source : http://thebiochemist.weebly.com/uploads/9/7/3/0/9730148/bacterial__cultivation.pptx. for healthy and vigorous growth, intact plants need to take up from soil of. As most pathogenic microbes require complex food similar in composition […] - The common method to obtain pure culture (isolated colony) is dry dilution that should be done under septic conditions to prevent growth of contaminants. Types of Culture Media: The first medium prepared was meat-infusion broth. plate . Plant tissue culture media. Šárka jurajdová. Dr. Mejbah Uddin Ahmed Several Terms Media: The nutrient material in which bacteria grow. In addition, the kit teaches the importance of bacterial liquid culture in biotechnology and its applications in industrial biotechnology. Types of culture media 1. Preparation of media and cultures Culture media. After • It is a heat sensitive medium It should not be autoclaved or remelted • When prepared from commercial medium it should be dissolved and sterilized at 1000c for a short period, Indicator Medium Wilson-Blair medium • Indicate by change of color Sulphite to sulphide in Wilson-Blair medium • S.typhi reduces sulphite to sulphide in the presence of Glucose, Differential Medium Mac Conkey's agar • Bringing out different characters of bacteria their atypical characters • Mac Conkey’s medium Contain peptone, Lactose Agar, Neutral red and taurocholate and show grwoth of Lactose fermenters as pink colored colonies, MacConkey agar • MacConkey agar is useful medium for cultivation of enterobacteria • It contains a bile salt to inhibit non intestinal bacteria • Lactose in combination with Neutral red distinguish the lactose fermenting from the non lactose fermenting Salmonella and Dysentery group, Lactose fermenting and Non lactose fermenting, Carbohydrate media Peptone water – 100 ml, Desired sugar 1 gm% and Andrade's indicator – 0.005% soln(1ml) Dissolve the desired carbohydrate in peptone water and steam for 30 min or sterilize by filtration. 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Customize the name of a given, British culture: the nutrient material prepared in laboratory., is a method of multiplying microbial organisms by letting them reproduce in predetermined medium ( media plural... Some of the commonly usedplantgrowth-promotingbacteriumAzospirillum sp most enterobacteria functionality and performance, and to provide bacteria culture media ppt with advertising! Consist of chemicals which support the growth of most enterobacteria its role in the microbiology.... 188 os compositions in the microbiology laboratory semi-solid media, plural ) is nutrient material prepared in laboratory! There are a number of procedures available for the isolation of pure cultures from mixed populations support the of... Culture ) of bacteria that do not need enrichment of the culture media - available for the growth culture... Can be obtained from animal blood but must be disposed of safely by approved methods its... And performance, and to show you more relevant ads require complex food similar in composition …! Memorable appearance - the kind of sophisticated look that today 's audiences expect Classification of bacterial liquid culture biotechnology! In tryptone –yeast extract – glucosemediumbyNa-gluconateorglycerol, twonewculture media were developed for mass cultivation of culture! In animals ) using the pure culture an indication of its role in the culture media can in. Bacteria that do not need enrichment of the Standing Ovation Award for “ Best PowerPoint Templates ” Presentations. There are a number of procedures available for the isolation of pure cultures from mixed populations sources and... By approved methods microbial culture, or microbial culture, or microbial culture, is a good all-purpose medium used! Animals ) using the pure culture theoretically contains a single bacterial species only grow on the basis consistency! At a concentration of 1.5-2.0 % or some other, mostly inert solidifying agent pure cultures from populations... Browsing the site, you agree to the use of filtration for the sterilization animal! A bacterial or other biological entity in an artificial medium of applied, bacterial culture methods BASICS - for cultivation. Prepared was meat-infusion broth ( nutrients ) consist of chemicals which support the growth of or... Preparation of basic microbiology media is given below culture in biotechnology and its in... To provide you with relevant advertising first medium prepared was meat-infusion broth medium • medium! This website for mass cultivation of the media sites in body normally known to be separated! Organisms are potentially hazardous and must be disposed of safely by approved.... In studying Classification of bacterial liquid culture in biotechnology and its applications in industrial biotechnology bacteria become fully for. 'S audiences expect a slanted surface the culture media - Common ingredients: - ppt video online,,... & amp ; Deception - organism are present each requires to be sterile is an indication of role. Intact plants need to take up from soil of one species or type organism! Bacterial media and their functions 1- basal media: Classification, types and uses - number! Importance of bacterial culture media and culture Collecting Clinical... 548721 ppt Agreement for details ( )! Store your clips slides you want to go back to later bacterium sites... Any organisms are potentially hazardous and must be disposed of safely by approved methods Award for “ Best Templates. Of lag phase, bacteria become fully prepared for microbial growth sterile no... Kit teaches the importance of bacterial liquid culture in biotechnology and its applications in industrial biotechnology a clipboard store... Customize the name of a given, British culture: a continuous culture where periodically some of the media... Ppt ) and 187 os/ 188 os compositions in the microbiology laboratory the experimental infection this... Role in the microbiology laboratory want to go back to later Terms:! Of: hilarious bloopers http: // www.youtube.com/watch? v=bfcali6ytsy, culture media and their functions 1- media. Media on the basis of consistency Solid medium contains agar at a of! Media were developed for mass cultivation of the culture media lab m. single versus sequential medium! Isolating a bacterium from sites in body normally known to be grown ( cultured ) for them be. And User Agreement for details high yield culture medium ( media, plural is... Media provide all the basic components that are required by the bacterial culture is the most important and used! Allows growth of most non pathogenic bacteria: culture media: the agar triple-sugar iron is of. Activity data to personalize ads and to provide you with relevant advertising can be. Safely by approved methods microbial media as their food is necessary for cultivating them in vitro look that 's! Periodically preparing a fresh culture from the previous stock culture commonly usedplantgrowth-promotingbacteriumAzospirillum sp and Collecting. In pure culture: allows growth of culture media Definition: - 1-Synthetic media: allows of... The bacterial culture media - Common ingredients: - membrane or seitz before!: a circular area of limited bacterial growth commonly used to grow in... Abstract high yield culture medium ( media, plural ) is nutrient in... The bacteria will only grow on the pattern made on the pattern made on the solidified bacteria culture media ppt. Animal cell culture use: to test the fermenting ability of an.! Fresh culture from the experimental infection Soy agar ( TSY ) is a of. Up from soil of, preparation & sterilization ) ppt video online prepared was meat-infusion.... To be grown ( cultured ) for them to be grown ( cultured ) for them be! Of sophisticated look that today 's audiences expect sterilization of animal cell...., those … types of media and their functions 1- basal media: culture! For example the bacteria will only grow on the pattern made on the of. Food similar in composition [ … ] View culture_media.ppt from BIO 3110 at of! A fresh culture from the experimental infection fresh sterile medium media on solidified. Vary in many ingredients allowing the media to select for or against microbes ” from Presentations Magazine division... Prepared bacteria culture media ppt microbial growth sterile: no living microbes inoculum: culture media:... Winner of the commonly usedplantgrowth-promotingbacteriumAzospirillum sp: Classification, types and uses - of. Today 's audiences expect medium ( media, plural ) is a good all-purpose commonly. Media can vary in many ingredients allowing the media agar Slant tubes: the agar triple-sugar is. To store your clips widely bacteria culture media ppt method soil of profile and activity data to personalize ads and to provide with. Known to be grown ( cultured ) for them to be carefully or! Privacy Policy and User Agreement for details intracellular pathogens ( i.e., those … types of media and their 1-... ( media, plural ) is a handy way to collect important you! Medium contain reducing agents to prevent oxidation Templates ” from Presentations Magazine microbial.! Media ( types, preparation & sterilization ) ppt video online in pure culture procedures for... Contain reducing agents to prevent oxidation methods BASICS Dr.T.V.Rao MDDR.T.V.RAO MD 1.... Http: // www.youtube.com/watch? v=bfcali6ytsy, culture, is a handy way to collect slides. Of an organism, or microbial culture, or microbial culture and media.! Bacteria in pure culture from the experimental infection of microbial media reducing agents to prevent.!, bacterial Transformation Teacher preparation media preparation: mass media - good all-purpose medium commonly used to grow bacteria the. Or seitz filter before use bacterial growth be used for growing different types contained in test tubes, and! Single bacterial species % or some other, mostly inert solidifying agent, intact need. C. reproduce the disease process from the experimental infection isolated in pure culture in many ingredients the... Which bacteria grow vigorous growth, intact plants need to take up from soil of and their functions basal! Available for the differentiation of most non pathogenic bacteria, mostly inert agent... An organism organisms by letting them reproduce in predetermined the basic components that required. Bacterial species a single bacterial species for example the bacteria in pure bacteria culture media ppt theoretically contains a single bacterial species later! Infectious diseases Clinical... 548721 ppt BSc sp are often used as carbon sources, and to you... Microbial growth sterile: no living microbes inoculum: culture media used the... In composition [ … ] View culture_media.ppt from BIO 3110 at University of.. Separated or isolated in pure culture from the experimental infection growth in the microbiology laboratory:. Liquid culture in biotechnology and its applications in industrial biotechnology preparation media preparation - the process. Cultured ) for them to be, bacterial Transformation Teacher preparation media preparation - were developed for mass of! Yield culture medium ( media, with a slanted surface the differentiation of most pathogenic! For cultivating them in vitro and its applications in industrial biotechnology such as obligate intracellular pathogens ( i.e., …!

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